IIT Guwahati - Experimental Biotechnology by NPTEL

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(6 Ratings)

Offered byNPTEL
Public/Government Institute

Experimental Biotechnology by NPTEL
at
NPTEL
Overview

Offered by Indian Institute of Technology Guwahati and NPTEL

Duration	12 weeks
Total fee	Free
Mode of learning	Online
Difficulty level	Advanced
Official Website	Explore Free Course
Credential	Certificate

Experimental Biotechnology by NPTEL

Table of contents

Overview
Highlights
Course Details
Curriculum
Student Reviews

Experimental Biotechnology by NPTEL
at
NPTEL
Highlights

Offered by IIT Guwahati
Final score comprises of 25% of average of best 8 assignments and 75% of the proctored certification exam score out of 100
Enrollments start from 20 July 2020
Course conducted by Prof. Vishal Trivedi (IIT Guwahati alumni)
Enroll for free
Pay for Certification Examination

Experimental Biotechnology by NPTEL
at
NPTEL
Course details

Who should do this course?

PG/PhD

What are the course deliverables?

By the end of this course, student will be able to understand:
Basics of Good Lab practices
Understanding different analytical techniques and their applications
Specific Scientific questions and their solutions
Designing new experiments

More about this course

In the current MOOCs course we have put effort to briefly discuss different analytical techniques and their potential in solving the scientific problems. We are taking several scientific problems or questions which can be solved by using these techniques.

Experimental Biotechnology by NPTEL
at
NPTEL
Curriculum

Week 1: Basics of Laboratory Research:Good Lab Practices, Concept of buffering and Preparation of Solutions and Reagents

Week 2: Electrophoresis (Part 1):Basic Concept of Electrophoresis, performance of electrophoresis and its applications

Week 3: Electrophoresis:Horizontal Gel electrophoresis, discussion about scientific questions and related experiments to solve them utilizing electrophoresis

Week 4: Chromatography (Part I): Analytical techniques in purification of biomolecules; Column chromatography, HPLC

Week 5: Chromatography (Part II) : Analytical techniques in purification of biomolecules; Column chromatography, HPLC

Week 6: Chromatography (Part III) : Discussion about scientific questions and related experiments to solve them utilizing chromatography

Week 7: Immunology Techniques: Immunological tools, Antibody Generation and Purification, ELISA, Radial Immuno-diffusion, Western blotting and Immuno-precipitation

Week 8: Cell Biology Techniques (Part 1): Basics of cell-culture, isolation of cells from tissue and fractionation

Week 9: Cell Biology Techniques (Part 2): Immuno-localization, cell sorting, and discussion about scientific questions and related experiments to solve them utilizing cell biology techniques

Week 10: Molecular Biology (Part 1):Primer designing, Polymerase chain reaction, Different variants of PCR and their applications

Week 11: Molecular Biology (Part 2) Blotting Techniques, DNA and protein sequencing techniques and their applications

Week 12: Summary and Conclusions