Experimental Methods in Systems Biology
- Offered byCoursera
- Public/Government Institute
Experimental Methods in Systems Biology at Coursera Overview
Duration | 18 hours |
Total fee | Free |
Mode of learning | Online |
Official Website | Explore Free Course  |
Credential | Certificate |
Experimental Methods in Systems Biology at Coursera Highlights
- Shareable Certificate Earn a Certificate upon completion
- 100% online Start instantly and learn at your own schedule.
- Course 2 of 6 in the Systems Biology and Biotechnology Specialization
- Flexible deadlines Reset deadlines in accordance to your schedule.
- Approx. 18 hours to complete
- English Subtitles: French, Portuguese (European), Russian, English, Spanish
Experimental Methods in Systems Biology at Coursera Course details
- Learn about the technologies underlying experimentation used in systems biology, with particular focus on RNA sequencing, mass spec-based proteomics, flow/mass cytometry and live-cell imaging.
- A key driver of the systems biology field is the technology allowing us to delve deeper and wider into how cells respond to experimental perturbations. This in turns allows us to build more detailed quantitative models of cellular function, which can give important insight into applications ranging from biotechnology to human disease. This course gives a broad overview of a variety of current experimental techniques used in modern systems biology, with focus on obtaining the quantitative data needed for computational modeling purposes in downstream analyses. We dive deeply into four technologies in particular, mRNA sequencing, mass spectrometry-based proteomics, flow/mass cytometry, and live-cell imaging. These techniques are often used in systems biology and range from genome-wide coverage to single molecule coverage, millions of cells to single cells, and single time points to frequently sampled time courses. We present not only the theoretical background upon which these technologies work, but also enter real wet lab environments to provide instruction on how these techniques are performed in practice, and how resultant data are analyzed for quality and content.
Experimental Methods in Systems Biology at Coursera Curriculum
Introduction
Lecture 1 -Scope and Overview
Lecture 2 - Biological Model Systems
Lecture 3 - Experimental Perturbations
Lecture 4 - Measuring Nucleic Acids
Lecture 5 - Measuring Protein and Protein States
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Reading List
Quiz 1
Deep mRNA Sequencing
Lecture 1 - History of Sequencing
Lecture 2 - 2nd and 3rd Generation Sequencing
Lecture 3 - Illumina-Based mRNA Sequencing
Lecture 4 - Lab-Based Video
Lecture 5 - mRNA Sequencing Data Analysis
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Reading List
Quiz 2
Mass Spectrometry-Based Proteomics
Lecture 1 - Basics of Mass Spectrometry 1
Lecture 2 - Basics of Mass Spectrometry 2
Lecture 3 - Quantification in Proteomics
Lecture 4 - Lab-Based Video - Proteomics Day 1
Lecture 5 - Lab-Based Video - Proteomics Day 2
Lecture 6 - Lab-Based Video - Analysis
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Lecture Slides
Reading List
Quiz 3
Midterm Exam
Midterm Exam
Flow and Mass Cytometry for Single Cell Protein Levels and Cell Fate
Lecture 1 - Flow Cytometry 1
Lecture 2 - Flow Cytometry 2
Lecture 3 - Mass Cytometry
Lecture 4 - Cytometry Data Analysis
Lecture 5 - Lab-Based Video - Flow Cytometry - Acquisition
Lecture 6 - Lab-Based Video - Flow Cytometry - Analysis
Lecture 7 - Lab-Based Video - Mass Cytometry
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Reading List
Quiz 4
Live-cell Imaging for Single Cell Protein Dynamics
Lecture 1 - Fluorescence Microscopy
Lecture 2 - Types of Imaging
Lecture 3 - Visualizing Molecules in Living Cells: Fluorescent Tools
Lecture 4 - Quantification
Lecture 5 - Lab-Based Video
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Reading List
Quiz 5
Integrating and Interpreting Datasets with Network Models and Dynamical Models
Lecture 1 - Omics data and Network Model Analyses
Lecture 2 - Single Cell Time Course Data and Dynamical Model Analyses
Lecture 3 - Dynamical Model Case Study
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Reading List
Quiz 6
Final Exam
Final Exam
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