IIT Kanpur develops a testing kit to detect E coli in drinking water

Escherichia coli or E coli is a bacteria that normally lives in the intestines of healthy people and animals. Most types of E coli are harmless but a few strains can cause severe stomach cramps, bloody diarrhea and vomiting.

IIT Kanpur develops a testing kit to detect E coli in drinking water

A team of researchers at the Indian Institute of Technology (IIT) Kanpur has developed a novel water testing kit that is highly sensitive and can detect the presence of E.coli in drinking water. Escherichia coli or E coli is a bacteria that normally lives in the intestines of healthy people and animals. Most types of E coli are harmless but a few strains can cause severe stomach cramps, bloody diarrhea and vomiting, the institute said.

"The kit is very easy to use, with false-positive results being rare. It is cheap, provides high accuracy, as well as has technological advantages over similar currently available kits in the market," said the researchers who have conducted its successful field trials in Odisha.

"This E coli water testing kit will surely be a boon when it comes to tackling water contamination and detecting the presence of E coli. The low-cost and higher accuracy nature of the product will give it the required advantage over its peers in the market and will be a benchmark for the Make in India initiative," said Prof Abhay Karandikar, Director, IIT Kanpur.

The test is conducted using pre-prepared vials filled with chemicals and a chemical-infused gel for 24 hours before reading. The methodology uses a growth medium specific for Ecoli and detection of a marker enzyme produced by the E coli bacteria. A visual colour change from the enzyme-substrate interaction and a simple method thereof determines the presence or absence of bacteriological contamination in drinking water. The kit detects biomarkers secreted only by E coli bacteria and will not change the colour in presence of other commonly found bacteria in tropical soil or water.

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